Fig. 5: Sexually dimorphic Tei expression in fru-positive neurons was unraveled by the intersection of tei-GAL4 and fruFLP.

a–f At ~5 h APF, neurons within a single cluster in the anterior lateral protocerebrum (encircled with a broken-line frame and enlarged in insets) strongly express FruM (magenta) in males (e, f) but not females (b, c), and conversely, the same cells express tei-GAL4 (green) in females (a, c) but not males (d, f). g–l At ~12 h APF, all known fru-positive clusters, including mAL (encircled with a broken-line frame and enlarged in insets), express FruM (magenta) in males (k, l) but not females (h, i), and tei-GAL4 expression (green) is detected in not only females but also males, despite the fact that the number of tei-GAL4-positive cells is reduced in males. Scale bars represent 50 µm and 10 μm (for insets).