Fig. 6: Overexpression of FruM represses tei expression in the larval CNS. | Communications Biology

Fig. 6: Overexpression of FruM represses tei expression in the larval CNS.

From: teiresias, a Fruitless target gene encoding an immunoglobulin-superfamily transmembrane protein, is required for neuronal feminization in Drosophila

Fig. 6: Overexpression of FruM represses tei expression in the larval CNS.The alternative text for this image may have been generated using AI.

a–c The control CNS expresses a high level of Tei as detected by the GFP reporter for tei-GAL4 (a) or anti-Tei antibody immunoreactivity (b) with little expression of FruM (c). d–f Overexpression of FruBM (f; detected by anti-FruM antibody) downregulates Tei expression as visualized by the reduced staining intensity for tei-GAL4 expression (d) and anti-Tei antibody immunoreactivity (e). Scale bars represent 50 µm. g, h Courtship indices (g) and 1-h copulation success (h) of male flies with the genotypes indicated estimated when paired with a Canton-S wild-type female. Statistical differences were evaluated by the Kruskal–Wallis analysis followed by Steel-Dwass nonparametric multiple comparisons (g) and one-way ANOVA followed by Fisher’s exact probability test (h). ***P < 0.001, *P < 0.05.

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