Fig. 6: Rapid creation of a recombinant VACV using Cas9 selection.
From: Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool
a Schematic showing a rapid 3-step protocol to exchange mCherry in VACV-mCh for venus, including the steps taken to track selection. b Proportions of mCherry-positive (mCh+), double-negative (DN), double-positive (DP) and Venus-positive viruses (Venus+) following homologous recombination and Cas9 selection are shown. One hundred plaques were counted per condition. Pie charts and adjacent numbers are means across two independent experiments.
