Fig. 1: Submergence inhibits wound responses.

Ten-day-old seedlings were used. Error bars indicate ±SD. Letters indicate groups that are statistically significantly different from each other (P < 0.05, Tukey’s test). a Effects of submergence on wound responses. Cotyledons and leaves of the Col-0 seedlings were left in the air (air) or transferred into the water (sub), wounded immediately and then harvested after 1 h incubation. Three biological replicates were averaged. b Re-aeration does not recover wound responses. The Col-0 seedlings were wounded in the water immediately after submergence and incubated for 1 h. Submerged seedlings were then re-aerated for 1 h before they were harvested (Sub re-air) or were left in water for another hour before harvest (Sub). Three biological replicates were averaged. c Submergence inhibits wound responses of re-aerated seedlings. The Col-0 seedlings were submerged for 1 h without wounding and then transferred to air. Re-aerated seedlings were immediately wounded and then harvested after 1 h. Three biological replicates were averaged. d Promoter activity of the JAZ10 gene. Ten-day-old pJAZ10:GUS transgenic seedlings were submerged and wounded as described in c. Seedlings were fixed with acetone 1 h after the wounding. Intensities of GUS signals were measured using ImageJ software. Four biological replicates were averaged. Scale bars indicate 0.1 cm. e Time-course expression of genes after wounding. Seedlings were submerged for 1 h and then transferred to air. Re-aerated seedlings were wounded and then harvested at the indicated time points. Three biological replicates were averaged. f Effects of submergence time on wound responses. Seedlings were submerged for the indicated time points and then transferred to air. Re-aerated seedlings were wounded immediately and then harvested after 1 h. Three biological replicates were averaged.