Fig. 2: Submergence inhibits JA biosynthesis.
From: Submergence deactivates wound-induced plant defence against herbivores
Ten-day-old seedlings were used. Error bars indicate ±SD. Letters indicate groups that are statistically significantly different from each other (P < 0.05, Tukey’s test). a Expression of JA-responsive genes after MeJA treatment. The Col-0 seedlings were submerged for 1 h and then transferred to the air. Re-aerated seedlings were treated with 25 μM MeJA. Whole seedlings were harvested 1 h after the treatment. Three biological replicates were averaged. b JA biosynthesis pathways. 13(S)-HPOT, (9Z,11E,15Z,13S)-13-hydroperoxy-9,11,15-octadecatrienoic acid; 12-oxo-PDA, 12-oxo-10,15(Z)-octadecatrienoic acid; OPC-8:0, 3-oxo-2(2′(Z)-pentenyl)-cyclopentane-1-octanoic acid. c Expression of JA biosynthesis genes after the submergence. The Col-0 seedlings were submerged and wounded as described in Fig. 1c. Three biological replicates were averaged. d JA accumulation after the wounding. The Col-0 seedlings were submerged and wounded as described in Fig. 1c. Five biological replicates were averaged. e Herbivore-feeding assay. Four-week-old Col-0 plants were submerged for 1 h and then transferred to the air. Two caterpillars (larval stage L1) of the Brassicaceae specialist P. rapae were released onto a single reaerated 4-week-old plant. Larval weights were measured before and 5 days after the caterpillar feeding (n = 8~24). The box plot shows median and quartiles. Scale bars indicate 0.5 cm.
