Fig. 2: The HACS1–PIRB interaction. | Communications Biology

Fig. 2: The HACS1–PIRB interaction.

From: HACS1 signaling adaptor protein recognizes a motif in the paired immunoglobulin receptor B cytoplasmic domain

Fig. 2

(a) Schematic representation of the PIRB receptor. The cytoplasmic region with four ITIM repeats spans approximately 190 residues. (b) Western detection of the HACS1-PIRB interaction. A pulldown was performed with immobilized HACS1 SH3 domain fragments fused to maltose-binding protein (MBP) and extracts of mouse bone marrow (BM) or spleen. Chemiluminescent detection of PIRB was achieved with a mouse anti-PIRB antibody.

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