Fig. 5: METH treatment induces cardiac fibrotic remodeling in mice. | Communications Biology

Fig. 5: METH treatment induces cardiac fibrotic remodeling in mice.

From: Methamphetamine induces cardiomyopathy by Sigmar1 inhibition-dependent impairment of mitochondrial dynamics and function

Fig. 5

Histopathological staining and immunoblot analysis showed increased cardiac fibrosis and enhanced expression of protein markers of fibrosis in the hearts of METH-treated mice compared with vehicle-treated mice. a Left panel: representative micrographs of Masson’s Trichrome stained LV sections in vehicle- and METH-treated mice. Right panel: quantification of the percent myocardial fibrosis area in the vehicle-treated (n = 5), and METH-treated (n = 6) mice. Scale bar: 50 µm. b Left panel: representative micrographs of Picro Sirius Red-stained LV heart sections in vehicle- and METH-treated mice. Right panel: quantification of the percent collagen area in the vehicle-treated (n = 5) and METH-treated (n = 6) mice. Scale bar: 50 µm. c Left panel: representative immunoblot images of periostin and α-smooth muscle actin; right panel: densitometric quantification of protein bands in the hearts of vehicle- and METH-treated mice. GAPDH was used as a loading control. Boxes depict interquartile ranges, lines represent medians, and whiskers represent ranges. P values were determined using a two-tailed unpaired Student’s t test. P < 0.05 between groups was considered statistically significant. Veh vehicle, METH methamphetamine.

Back to article page