Fig. 6: METH alters the expression of mitochondrial dynamics regulatory protein Fis1 in the heart.

a, b Representative immunoblot images and densitometric quantification of the expression of mitochondrial fission, i.e., Drp1, Fis1, Mff, and fusion, i.e., OPA1, OMA1, Mfn2, regulatory proteins in the whole-cell a and isolated mitochondrial b fractions from vehicle- and METH-treated mouse hearts. GAPDH was used on the same membrane of whole-cell and mitochondrial fractions to assess the purity of isolation of mitochondria. Mitochondrial outer membrane integral protein, Tom20, and mitochondrial inner membrane integral protein, Tim23, were used to confirm mitochondrial extracts. Ponceau S staining of the transfer membranes was used to verify approximately equal loading and transfer across the gel. Bar graphs represent mitochondrial dynamics regulatory protein expression changes in the hearts of METH-treated mice compared with those of vehicle-treated mice (n = 3–10 mice per group). Boxes depict interquartile ranges, lines represent medians, and whiskers represent ranges. P values were determined using a two-tailed unpaired Student’s t test. P < 0.05 between groups was considered statistically significant. Veh vehicle, METH methamphetamine, NS not significant.