Fig. 1: Main experimental design in the study.

a Representation of the NuTRAP construct and its recombined products upon cre-mediated induction. b Breeding strategy to achieve astrocyte- and microglia- specific RNA and DNA. c Schematic of the cell-specific RNA and DNA isolation from the same mouse brain via the TRAP and INTACT methods. Note: currently TRAP and INTACT from the same homogenate is not possible, but parallel structures from each hemisphere of the same mouse can be used for paired analyses.