Fig. 1: Tudor protein is expressed in perineurial, subperineurial, and cortex glia.
From: Glial granules contain germline proteins in the Drosophila brain, which regulate brain transcriptome
a GFP-tagged endogenous Tud (green channel) is expressed in glial cells (indicated with arrows, glia nuclei labeled with anti-Repo antibody, magenta) in the adult brains. b A diagram of the fly adult brain with surface and cortex glia subtypes indicated. A cortex glial cell envelops multiple neuronal bodies forming a honeycomb-like architecture. Nuclei of two cortex glial cells are shown in black. General area of the central brain imaged in c–h is indicated with a box. c High-magnification super-resolution optical section shows Tud localization (green) to glia (Repo-labeled, magenta). d–f Low-magnification optical sections show FLAG-tagged Tud localization (magenta) to perineurial (d), subperineurial (e), and cortex (f) glia. Different glia subtypes were labeled with the membrane marker GFP-mCD8 (green). g, h 3D high-magnification images of Tud granules (magenta) assembled in perineurial (g) and cortex (h) glia labeled with the membrane marker GFP-mCD8 (green). These images are composites of 186 optical sections (perineurial glia) and 225 sections (cortex glia) obtained with super-resolution confocal microscopy. Scale bars in a is 100 μm; in c is 5 μm; in d–f are 20 μm; and in g, h are 10 μm.
