Fig. 5: Immune population and cytokine analysis of tumors.
From: Inhibition of the CCL2 receptor, CCR2, enhances tumor response to immune checkpoint therapy
Flow cytometry-based analysis of (a) LAG3 and (b) PD-1 expression levels on CD8+ T cells from E0771 tumors. Frequency of (c) FoxP3+ CD4+ and (d) CD8+ T cells of tumor-infiltrating lymphocytes. e Cytokine array of tumor tissue harvested 25 days post subcutaneous E0771 injection into mice. Data are presented as mean pixel density as determined on the membrane by chemiluminescence with Biorad ChemiDoc MP Imaging system. f Number of IFNγ+ CD8+ T cells per gram of tumor tissue. All dot plots are presented as mean ± SD and box plots as mean with min/max. Statistical significance was determined by two-way ANOVA.
