Fig. 2: The NHE1-LID binds different lipids with induction of helical structures. | Communications Biology

Fig. 2: The NHE1-LID binds different lipids with induction of helical structures.

From: The intracellular lipid-binding domain of human Na+/H+ exchanger 1 forms a lipid-protein co-structure essential for activity

Fig. 2

a Position of individual lipids on the dot blot membrane. b Lipid binding profile of the CHP1/NHE1503-595 and CHP1/NHE1503-698 at pH 7.4 and 8.4. c Variants of NHE1 used to test lipid binding specificity. d Effect of various mutations on CHP1/NHE1503-595 lipid binding. e Far-UV CD spectra of NHE1-LID539-593 in DHPC detergent (color) and in DMPC:DHPC bicelles (dashed color). The CD spectrum of NHE1-LID539-593 in water is shown in black. f Far-UV CD spectra of the NHE1-LID in anionic bicelles consisting of DMPG:DMPC:DHPC (dashed color) and in 2% LPPG (color). The CD spectrum of NHE1-LID539-593 in water is shown in black. g 15N,1H-HSQC spectrum of NHE1-LID539-593 in 2% LPPG, 320.15 K. h SCSs of Cα of the NHE1-LID in 2% LPPG with two highly populated helices, H1 and H2, indicated below the sequence.

Back to article page