Fig. 2: Reduced miR-210 expression in diabetic wounds.
From: HypoxamiR-210 accelerates wound healing in diabetic mice by improving cellular metabolism
a, b miR-210 expression levels were analyzed in skin (n = 10) and wounds (n = 9) from normoglycemic wild-type (WT) mice, as well as in wounds from db/db diabetic mice (n = 10) using in situ hybridization (a) and quantitative RT-PCR (b). c, d Wound biopsies from diabetic foot ulcers (DFU, n = 8) and venous ulcers (VU, n = 7) were analyzed for miR-210 expression using in situ hybridization (c) and quantitative RT-PCR (d). Scale bar = 50 μm. HIF-1α expression was analyzed by immunofluorescence (e) and miR-210 expression levels by quantitative RT-PCR (f) in wounds from WT-control mice (n = 8) and wounds from db/db mice treated with placebo (n = 4) or with DMOG (n = 4). Scale bar = 100 μm. Statistical differences were calculated using one-way ANOVA (b, f) and Student’s t test (d). Data are represented as mean ± s.e.m. *P < 0.05.
