Fig. 5: Metabolic reprogramming by miR-210 improves fibroblasts function.

HDF cells were transfected with control mimic or miR-210 mimic and exposed to hypoxia and high glucose concentration (30 mM) followed by measurement of a gene expression levels of ISCU (n = 6) and SDHD (n = 5), b oxygen consumption rate (OCR) (n = 6), c extracellular acidification rate (ECAR) (n = 4), d ROS levels (n = 5) and e mitochondrial and glycolytic ATP production rate (n = 5). f Migration of HDF that were treated with vehicle or glycolysis inhibitors 2-deoxy-d-Glucose (2-DG, 15 mM), Syrosingopine (Syro, 10 µM), and Oxamate (45 mM) along with hypoxia and high glucose concentrations (n = 6). Statistical differences were calculated using paired Student’s t test. Wilcoxon matched-pairs signed rank test was used for Syrosingopine-treated groups where the data were not normally distributed. Data are represented as mean ± s.e.m. *P < 0.05.