Fig. 2: The binding strength of single agglutinins.
From: Single-cell fluidic force microscopy reveals stress-dependent molecular interactions in yeast mating
a Maximum adhesion force histograms obtained by recording force–distance curves in PBS between three independent MATa and MATα cell pairs pretreated with pheromones, at an applied force of 1 nN, with a probing time of 1 s and a retraction velocity of 1000 nm s−1. The corresponding adhesion probability (Padh) is mentioned in each histogram. Gaussian fits for a multimodal distribution are overlapped with the raw histogram: in colored plain lines, the independent Gaussians and in dashed black lines the overall fit. b Representative retraction force profiles color coded according to the peaks identified in the distributions. c Box plots of the mean adhesion forces revealed by the multi-peak center analysis, as illustrated in a. Stars are the mean values, lines the medians, boxes the 25–75% quartiles, and whiskers the SD from N = 7 independent cell pairs. d Rupture length histogram obtained by merging the data obtained on the three cell pairs shown in a.
