Fig. 3: The role of applied force and probing time in cell–cell adhesion.
From: Single-cell fluidic force microscopy reveals stress-dependent molecular interactions in yeast mating
a Box plots of the adhesion probability between MATa and MATα cells, pretreated with pheromones, recorded under different applied forces (1 or 0.25 nN) and with different probing times during force–distance curve measurements (1 or 0.1 s). Stars are the mean values, lines the medians, boxes the 25–75% quartiles, and whiskers the SD from N = 5 independent cell pairs. b Scatter plots, overlapped with data points, of the adhesion forces reached at diverse quartiles (Q1: 25%, median: 50% and Q3: 75% of the adhesive population) as a function of the applied force and probing time used to record the force–distance curves between MATa and MATα cells. Error bars are the SD from N = 6 independent cell pairs. Dashed lines are a guide for the eye. c Proportion of the first peak identified in Fig. 2c (vs the total adhesive population) as a function of the same parameters. Error bars are the SD from N = 5 independent cell pairs.
