Fig. 2: Application of smSBS to a small quantity (~10 pg) of cDNA library of K562 cells.

a Schematic illustration for the capture and sequence of unfragmented full-length cDNAs. Lines in the cDNA molecules indicate TSO (red solid), complementary of TSO (red dashed), polydT (yellow solid), and poly-A (yellow dashed). b Read length distribution after 24Q sequencing; reads <19 nt and containing byproducts were filtered out. c Venn diagram of the number of genes detected with smSBS and an Illumina sequencer (Hiseq2500). Genes detected with smSBS and Illumina were counted as HTseq-count > 0 and TPM of RSEM > 0, respectively. d Comparison of gene expressions obtained via smSBS and Illumina. e Normalized density plot of gene length detected via smSBS and Illumina. f Gene body coverage via smSBS and Illumina. Note that, the Illumina results shown in c–f were calculated with the reads down-sampled into 200k paired-end reads.