Fig. 3: Mitochondrial function assays in MMC under normal and high-glucose conditions.

a Seahorse XF Cell Mito Stress test for mitochondrial function at basal conditions, ATP production, and maximal respiration levels using MMC from WT or miR-379KO mice cultured with normal glucose (NG, 5.5 mM) or high glucose (HG, 25 mM) for 72 h. b Oxygen consumption rates (OCRs) were calculated in basal and spare respiratory capacity (SRC) levels in NG and HG condition (20,000 cells per well of 96-well assay plate). c Representative images of IHC staining to detect FIS1 expression (brown color) in negative control (NC) and Fis1 siRNA (si-Fis1)-transfected MMC from WT or miR-379KO mice cultured with NG or HG. d Bar graph quantifications showing significant reduction in FIS1 levels in si-Fis1-transfected NG- or HG-treated WT and miR-379KO MMC compared to NC, and significant decrease in FIS1 levels in HG-treated WT MMC but not miR-379KO MMC (n = 15 cells/group). The in vitro experiments were performed with at least three biological replicates. One-way ANOVA with Tukey’s post hoc test for multiple comparisons. **P < 0.01, ****P < 0.0001. All data are presented as mean ± SEM. NC, negative control siRNA. si-Fis1, Fis1 siRNA.