Fig. 2: Anti-biofilm activity of cannabidiol.
Minimum biofilm eradication concentration (MBEC) assessment of CBD using crystal violet staining (a) to assess (b) MSSA ATCC 25923 and MRSA ATCC 43300 biofilm remaining after 24 h incubation with CBD, vancomycin, daptomycin, trimethoprim, mupirocin, or clindamycin (biofilm initially established by 48 h growth in TSB + 5% glucose). Data are n = 4 biologically independent samples. c Confocal microscopy of MRSA ATCC 43300 biofilm grown on microscope slides, then treated with CBD at varying concentrations for 24 h. Slides were then stained with SYTO 9 (green, indicates live + dead cells) and propidium iodide (red, indicates dead cells) nucleic acid stains (white scale bars represent 20 µm in the 3D panels and 5 µm in the 2D panels).
