Fig. 4: Analysis of circulating innate immune cells after LASV challenge.

a The number of leukocytes, granulocytes, mDC (HLA-DR⁺ CD14⁻ CD1c⁺ and HLA-DR⁺ CD14⁻ CD11c⁺), and monocytes (HLA-DR⁺ CD14⁺) in the blood is presented according to the time after LASV infection. The percentage of CD10⁻ cells among granulocytes is also presented. The percentage of monocytes expressing CD80, CD86, or CD40 is shown. Results show the mean ± standard error of the mean (SEM) for each group: controls (n = 3), LASV-Josiah (n = 6, except for day 14 where n = 2) and AV-infected animals (n = 4) were analyzed for leukocyte, granulocyte, CD10⁻, and monocyte numbers. For mDC, CD80, CD86, and CD40 analysis, six AV-infected animals were analyzed from day 0 to 6 and three of them from day 8 to 11. b The number of circulating NK cells (CD8⁺ CD3⁻ CD20⁻ cells) is presented, as well as the percentage of KI67⁺, CD107a⁺, NKp80⁺, and NKG2D⁺ cells among NK cells (n = 3 for controls, n = 6 for LASV-Josiah, and n = 4 for AV-infected animals). Statistical analyses were performed and are presented as in Fig. 1. Individual values can be found in Supplementary data 1 for a and b. c The proportion of NK cells (CD8⁺ CD3⁻ CD20⁻) expressing KI67, (granzyme B) GrzB, CD107a, CXCR3, and NKp80 was quantified in spleen (S, upper graphs) and MLN (L, lower graphs) of controls (n = 3), AV- (n = 3), and Josiah-infected (n = 3) animals, as well as the percentage of CD16⁺ CD56⁻ cells among NK cells. Individual values and mean ± SEM are expressed for each group. Statistical analyses were performed and are presented as in Fig. 1.