Fig. 5: Analysis of B-cell responses after LASV challenge.
a The number of B cells (CD20+) in the blood is presented according to the time after LASV infection. Results show the mean ± SEM of control (n = 3), AV (n = 4), and Josiah (n = 6) animals. b Detection of LASV-specific IgM and IgG in animals after LASV infection by ELISA. Individual data (points) and mean values ± SEM for each cohort (curves) are presented. LASV-AV (n = 4), LASV-Josiah (n = 6). **p < 0.01, ***p < 0.001, as determined by a Student’s t test. The percentage of naive/unconventional memory (N/UM) B cells (CD20+ CD38mid CD27− CD10−), of conventional memory (CM) B cells (CD20+ CD38mid CD27+ CD10−), and of plasma B cells (CD20low CD38bright CD27+) among B cells (CD20+) is presented, as well as the percentage of KI67+ cells among N/UM and CM B cells is presented for blood c and spleen (S, upper graphs) and MLN (L, lower graphs). Individual values can be found in Supplementary data 1 for a, b and c. d The percentage of KI67+ among transitional memory (TM) B cells (CD20+ CD38mid CD10+ CD27−) is also presented for SLOs. Results show the mean ±SEM of control (n = 3), AV (n = 4 for PBMC and three for SLOs), and Josiah (n = 6 and three for SLOs) animals, as well as individual values. Statistical analyses were performed and are presented as in Fig. 1.
