Fig. 2: CtNatB acetylation activity and inhibition.
From: Structural basis of Naa20 activity towards a canonical NatB substrate
a Substrate specificity of CtNatB tested with five different peptides. SESS, EEEI, MDEL, MLGTE and MVNALE were previously identified as NatA, Naa10/Naa80, NatB and NatC/E/F substrates. b Michaelis–Menten curve of the CtNatB mediated acetylation of MDEL. c Structure of the bisubstrate analog CoA-Ac-MDEL. d Dose-response curve of the NatB mediated acetylation, inhibited by CoA-Ac-MDEL. e Inhibitor characteristics of CoA-Ac-MDEL compared to other bisubstrate analogs32,41. All reactions were performed in triplicates and error bars represent the standard deviation.
