Fig. 5: Characterization of CtNatB mutants.

a The stability of different NatB mutants (mutations in the catalytic subunit CtNaa20_1-166) was assessed by nanoDSF in triplicates and error bars represent the standard deviations. b The wild-type efficiencies of different point mutants were calculated from k_cat and K_m values, determined in individuals experiments in triplicates. The data represent the mean values with standard deviations of these experiments, normalized to the wild-type value and considering the propagation of uncertainty. c Superimposition of mutated residues with the corresponding CaNaa20 residues. CtNaa20 is shown as transparent cartoon with catalytically important residues shown as red sticks. The corresponding CaNaa20 residues (pdb: 5k18³⁰) are shown as cyan sticks and CoA-Ac-MDEL is shown as gray sticks.