Fig. 7: The NGF interacts with CHRM4. | Communications Biology

Fig. 7: The NGF interacts with CHRM4.

From: Nerve growth factor interacts with CHRM4 and promotes neuroendocrine differentiation of prostate cancer and castration resistance

Fig. 7

a Immunoprecipitation (IP) of the NGF and Western blotting of CHRM4 and the NGF in LNCaP and C4-2 cells cultured in charcoal stripped serum (CSS)-containing medium for 1 week. b IP of CHRM4 and Western blotting of CHRM4 and the NGF in LNCaP and C4-2 cells cultured in CSS-containing medium for 1 week. c IP of CHRM4 and Western blotting of CHRM4 and the NGF in LNCaP cells following stable NGF-knockdown and culturing in CSS-containing medium for 1 week. d IP of the NGF and Western blotting of CHRM4 and the NGF in PC3 and NCI-H660 cells stably expressing a non-target control (NC) or NGF shRNA vector. e IP of CHRM4 and Western blotting of CHRM4 and the NGF in PC3 and NCI-H660 cells stably expressing the NC or NGF shRNA vector. f, g IP of the NGF f or Flag g and Western blotting of CHRM4 and the NGF in a mixture of recombinant NGF protein and in vitro transcription/translation synthesized Flag-CHRM4 protein. h Proposed model of ADT or AR inhibitor-mediated therapeutic resistance and neuroendocrine differentiation of prostate cancer cells by stimulation of the ZBTB46 transcription factor, which upregulates the NGF. The upregulated NGF promotes ADT-resistance and NEPC development by integrating CHRM4 and activating the AKT–MYCN pathway.

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