Fig. 3: Differential baseline BCL-2 family gene expression in adult, pediatric and fetal OL-lineage cells.

a–c Relative expression of pro and anti-apoptotic BCL-2 family genes across OL lineage spectrum in relation to age and lineage. Scale is z-scores of averaged normalized gene expression across the cell types or age groups; percent expressed indicates the percentage of OL-lineage cells in the category on the x-axis expressing the gene. Arrows indicate up- (orange) or down- (blue) regulation (adj. p < 0.05) against all other groups, where one arrow |logFC | > 0.1, two arrows |logFC | > 0.25, by Wilcoxon rank sum differential expression testing with Bonferroni correction. d Data indicates the ratios of average expression of individual pro- and anti-apoptotic genes for mature OLs. Data under all pediatric are combined from pediatric >5 and pediatric <5. Open circles indicate outliers by Grubbs’ test. Comparison of summated and individual ratios between adult and all pediatric donors was done by two-way ANOVA with Sidak’s correction for multiple comparisons both with and without the outliers, *p < 0.05. Age group comparisons of summated and individual ratios between adult, pediatric >5, and pediatric <5 was done by two-way ANOVA with Tukey’s correction for multiple comparisons, both with and without the outliers, *p < 0.05, **p < 0.01. Number of independent biological samples by age group: 4 adult, 7 pediatric (3 pediatric >5, 4 pediatric <5) and 4 fetal.