Fig. 3: Analysis of motility in standard IVMA buffer and optimized TIRF buffer. | Communications Biology

Fig. 3: Analysis of motility in standard IVMA buffer and optimized TIRF buffer.

From: Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms

Fig. 3

a Actin gliding velocity produced by HMM adsorbed to TMCS derivatized glass surfaces. In each individual experiment, 15 actin filaments were analyzed. b Actin gliding velocity produced by HMM adsorbed to TMCS derivatized glass surfaces. In each individual experiment, 25 (IVMA buffer) and 20 (IVMA buffer with TX/TQ) actin filaments were analyzed. c Fraction of motile filaments from the experiments under a, analyzed from five recordings of, in total, 1004 filaments (IVMA buffer) and 925 filaments (TIRF buffer), respectively. Data are given as mean ± 95% confidence interval. Temperature 26 ± 2 °C. For details about buffer composition please see Supplementary Information sections 1.2 and 1.9).

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