Fig. 4: Cumulative dwell time distributions recorded from different individual actomyosin hotspots to illustrate the heterogeneity in time constants. | Communications Biology

Fig. 4: Cumulative dwell time distributions recorded from different individual actomyosin hotspots to illustrate the heterogeneity in time constants.

From: Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms

Fig. 4

a Cumulative frequency distributions of Alexa-nucleotide dwell time events comparing actin-activated ATPase activity from four different hotspots. Data are fitted with double-exponential functions. b Amplitudes and rate constants obtained from fittings to data in a. Note that from the individual actomyosin hotspots only two processes attributed to actin-activated ATPase and “unexplained” phase can usually be resolved, while basal myosin ATPase remains obscured. For hotspot 2, it seems likely that the binding site of the HMM molecule is unfavorable for actin attachment, explaining a high ratio of basal to actomyosin ATPase. Error estimates refer to 95% confidence intervals derived in the regression analysis. Temperature: 20 °C.

Back to article page