Fig. 1: Sperm viability, sperm counts, ovary mass, and viral metrics.

See Table 1 for a complete summary of statistical tests, parameters, and p values. A Experimental schematic. B imported, C healthy, and D failed queens were surveyed for macroscopic health metrics. Symptoms shown are as reported by donating beekeepers. Sperm viability and sperm count data were acquired for 125 queens, while 123 queens contributed to final proteomics data owing to sample loss during handling. *** indicates p < 0.005. (E) For a subset of the queens, viral copy numbers were measured using RT-qPCR (n = 44 healthy queens, n = 13 imported queens, and n = 49 failed queens). Lower-case letters indicate statistical significance (p < 0.05) within each virus.