Fig. 5: TNF-induced complex I formation is not altered in WT and MIB2 KO cells. | Communications Biology

Fig. 5: TNF-induced complex I formation is not altered in WT and MIB2 KO cells.

From: MIND bomb 2 prevents RIPK1 kinase activity-dependent and -independent apoptosis through ubiquitylation of cFLIPL

Fig. 5: TNF-induced complex I formation is not altered in WT and MIB2 KO cells.The alternative text for this image may have been generated using AI.

a WT and MIB2 KO HeLa cells were stimulated with GST-TNF (1 μg/ml) for the indicated times, and TNFR-containing complex I was precipitated with glutathione-Sepharose. Precipitated proteins were analysed by immunoblotting with the indicated antibodies. Protein expression was verified by immunoblotting with the indicated antibodies. b WT and MIB2 KO HeLa cells were stimulated with TNF (10 ng/ml) for the indicated times, and cell lysates analysed by immunoblotting with the indicated antibodies. Asterisks indicate cross-reacted bands. All results are representative of two or three independent experiments.

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