Fig. 1: Exposure to a blood meal containing DENV-2 or ZIKV induces DNA fragmentation in the midgut epithelium of adult female Aedes aegypti (ORL) mosquitoes at 2hpi. | Communications Biology

Fig. 1: Exposure to a blood meal containing DENV-2 or ZIKV induces DNA fragmentation in the midgut epithelium of adult female Aedes aegypti (ORL) mosquitoes at 2hpi.

From: Clustered rapid induction of apoptosis limits ZIKV and DENV-2 proliferation in the midguts of Aedes aegypti

Fig. 1

a Workflow diagram of in vivo infection. b–d Representative images of DNA fragmentation visualized via TUNEL with DAPI counterstain in midguts from adult female ORL mosquitoes at 2 hours post b naive blood meal c ZIKV infected blood meal or d or DENV-2-infected blood meal. Scale bar shown is 100 µm in length and can be applied to all images. e Quantification of TUNEL-positive cells per midgut at 2 hours post in vivo infection (n (naive) = 12; n (ZIKV) = 17; n (DENV-2) = 24). The horizontal line indicates the median. Treatments without a common letter were found to be statistically significant (α = 0.05) as calculated by Kruskal–Wallis test with Mann–Whitney post hoc comparison (Kruskal–Wallis chi-squared = 32.017, df = 2, p value = 1.116e-07).

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