Fig. 3: Aedes aegypti MOYO-R strain mosquitoes that are partially resistant to DENV-2 have higher rates of TUNEL-positive cells in the midgut than DENV-2 susceptible MOYO-S strain mosquitoes at 2 hours post ingestion of a DENV-2-infected blood meal. | Communications Biology

Fig. 3: Aedes aegypti MOYO-R strain mosquitoes that are partially resistant to DENV-2 have higher rates of TUNEL-positive cells in the midgut than DENV-2 susceptible MOYO-S strain mosquitoes at 2 hours post ingestion of a DENV-2-infected blood meal.

From: Clustered rapid induction of apoptosis limits ZIKV and DENV-2 proliferation in the midguts of Aedes aegypti

Fig. 3

a–d Representative images of TUNEL with DAPI counterstain in midguts from adult female mosquitoes at 2 hours post blood meal. a MOYO-S mosquitoes fed naive blood, b MOYO-S mosquitoes fed DENV-2-infected blood meal, c MOYO-R mosquitoes fed naïve blood or d MOYO-R mosquitoes fed DENV-2-infected blood. Scale bar = 100 µm. e Quantification of TUNEL-positive cells per midgut at 2 hours post in vivo infection (n (MOYO-R naive) = 20; n (MOYO-R DENV-2) =  21; n (MOYO-S naive) = 23; n (MOYO-S DENV-2) = 24). The horizontal line indicates the median. Treatments without a common letter were found to be statistically significant (α = 0.05) as calculated by Kruskal–Wallis test with Mann–Whitney post hoc comparison (Kruskal–Wallis chi-squared = 41.907, df = 3, p value = 4.199e-09). Scale bar shown is 100 µm in length and can be applied to all images.

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