Fig. 1: Distribution of hypothalamus Lhx6-expressing neurons. | Communications Biology

Fig. 1: Distribution of hypothalamus Lhx6-expressing neurons.

From: Gene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons

Fig. 1

A, B, E–G In situ hybridization showing Lhx6 (blue) and Shh (brown) at E11.5 (A), E12.5 (B), E14.5 (E), shown in sagittal planes, and E16.5 (F, G) in coronal planes. Red arrows (A, B, E) indicate the intrahypothalamic diagonal (ID), red arrowheads (A, B, E) indicate the tuberomammillary terminal (TT), black arrows (D) indicate migrated telencephalic Lhx6-expressing cells (tangential migration from the medial ganglionic eminence to the cortex). Red arrows in (F) indicate the ZI and red circle in (F) indicates the DMH, and red arrows in (G) indicate the PH. C, D Schematics showing the distribution of telencephalic (green) and hypothalamic (purple) Lhx6-expressing cells at E11.5, with ID (red) and TT (blue) are highlighted in (D) (E11.5) Note anterior domains to the ID that shows a weak and transient Lhx6 expression during development (Ant.ID anterior ID, DMH dorsomedial hypothalamus). H scRNA-Seq from E11-E13 hypothalamus scRNA-Seq from24 showing the distribution of neurons that express GABAergic markers (blue, Dlx1/2, Gad1/2, and Slc32a1) and Lhx6-expressing GABAergic neurons (brown) that are ~2% of all hypothalamic GABAergic neurons during development. I Schematic distribution of Lhx6-expressing neurons across the dorsolateral hypothalamus (red = neurons that continue to express Lhx6, purple = neurons that transiently expressed Lhx6) across ZIv (zona incerta ventral), ZIl (zona incerta lateral), LH (lateral hypothalamus), DMH (dorsomedial hypothalamus), VMH (ventromedial hypothalamus), and PH (posterior hypothalamus). J–C′ Lhx6-antibody staining (gray), tdTomato expression from Lhx6Cre/+;;Ai9 line (red), NeuN (yellow) in ZIv (J–M), ZIl (N, Q), DMH (R–U), LH (V–Y), PH (Z–C′). L = lateral, M = medial. White arrowheads show neurons that transiently expressed Lhx6, and white arrows show neurons continue to express Lhx6). D′ A bar graph showing the percentage of tdTomato+ and Lhx6-expressing neurons over the total number of tdTomato+ neurons. Scale bar = 0.45 mm (A), 0.5 mm (B, F, G), 0.55 mm (E), 0.1 mm (I–C′). All bar graphs (D′) show mean and standard error of the mean (SEM), with individual data points plotted.

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