Fig. 1: Evaluation of MERS-CoV macro domain binding with NAD metabolites by Tm values.
From: Elucidating the tunability of binding behavior for the MERS-CoV macro domain with NAD metabolites
A Chemical structures of NAD metabolites tested in this study. Melting temperatures of 10 μM MERS-CoV macro domain in complex with 1 mM NAD metabolites were determined by B thermal shift assays and C circular dichroism. MERS-CoV macro domain in the apo form, ADPR-bound, NAD-bound, ATP-bound, ADP-bound, and AMP-bound form are labeled in black circles, green squares, orange triangles, yellow triangles, blue diamonds, and pink circles, respectively.
