Table 3 ITC measurements of NAD metabolites bound to MERS-CoV macro domain.
From: Elucidating the tunability of binding behavior for the MERS-CoV macro domain with NAD metabolites
Ligand bound at 298 K | n | Kd (μM) | ΔH (KJ/mol) | −TΔS (KJ/mol) | ΔG (KJ/mol) |
|---|---|---|---|---|---|
ADPR | 1.25 ± 0.08 | 3.67 ± 2.03 | −64.21 ± 4.00 | 32.85 ± 2.96 | −31.37 ± 1.69 |
NAD | 0.938 ± 0.13 | >1000 | 1.18 ± 1.09 | −15.9 ± 1.09 | −17.12 ± 0.00 |
ATP | 1.28 ± 0.23 | 289.30 ± 32.28 | −0.431 ± 0.26 | −19.77 ± 0.48 | −20.20 ± 0.28 |
ADP | 1.08 ± 0.06 | 70.29 ± 29.00 | −16.11 ± 7.54 | −7.77 ± 6.32 | −23.88 ± 1.23 |
AMP | 1.05 ± 0.38 | 191.23 ± 67.29 | −11.19 ± 3.02 | −10.12 ± 2.39 | −21.31 ± 0.81 |
Ligand bound at 308 K | n | Kd (μM) | ΔH (KJ/mol) | −TΔS (KJ/mol) | ΔG (KJ/mol) |
ADPR | 1.20 ± 0.19 | 26.92 ± 13.47 | −18.05 ± 18.45 | −9.30 ± 17.61 | −27.36 ± 1.33 |
NAD (WT) | 1.04 ± 0.12 | 54.34 ± 2.81 | −2.73 ± 3.66 | −22.59 ± 3.77 | −25.32 ± 0.13 |
NAD (G45V) | 1.31 ± 0.10 | 154.50 ± 42.19 | −76.05 ± 41.49 | 53.35 ± 41.50 | −22.70 ± 0.71 |
NAD (I129F) | ND | ||||
ATP | ND | ||||
ADP | ND | ||||
AMP | ND | ||||
