Table 1 Data obtained from Oxford Nanopore runs both mined and retrieved mitochondrial DNA (see text for protocol information).
Run | Modification to library preparation | Chemistry | Total # of reads (K) | Total yield (Gb) | mt-reads >500 bp | mtDNA yield (kb) | Mapped molecule 1 mt-reads | Mapped molecule 1 yield (kb) | Mapped molecule 2 mt-reads | Mapped molecule 2 yield (kb) |
|---|---|---|---|---|---|---|---|---|---|---|
1 | 10 kb shear | 1D, R9.4, SQK-108 | 165.43 | 0.99 | 7 | 25.31 | 7 | 25.31 | 0 | 0 |
2 | 10 kb shear, Trypsin | 1D, R9.4, SQK-108 | 353.58 | 2.06 | 16 | 94.42 | 9 | 43.87 | 5 | 24.54 |
3 | 2D | 2D, R9.4, SQK-208 | 18.79 | 0.26 | 10 | 27.83 | 9 | 45.02 | 0 | 0 |
4 | 5 kb shear | 1D, R9.4, SQK-108 | 34.64 | 0.31 | 0 | 0 | 0 | 0 | 0 | 0 |
5 | Trypsin | 1D, R9.4, SQK-208 | 265.17 | 0.98 | 8 | 46.46 | 6 | 14.16 | 1 | 4.836 |
6 | Trypsin | 1D, R9.4, SQK-108 | 18.31 | 0.11 | 1 | 3.43 | 1 | 3.39 | 0 | 0 |
7 | Trypsin | 1D, R9.4, LSK109 | 1224.00 | 1.30 | 36 | 74.99 | 31 | 64.60 | 4 | 8.22 |
8 | Trypsin | 1D, R9.4, LSK109 | 5149.57 | 3.45 | 80 | 112.71 | 51 | 74.69 | 22 | 26.09 |
Totals | 7229.48 | 9.46 | 158 | 385.15 | 114 | 271.04 | 32 | 63.69 | ||
