Fig. 5: Putative model of IRGB10 oligomerization-mediated membrane disruption.

a The putative membrane-bound regions of IRGB10. The blue dotted line indicates the N-terminus region where myristoylation occurs. The close-up view in the panel on the right shows the two putative IRGB10 transmembrane regions (pTMs). The close-up view of this area is shown in a panel on the right. pTM1 and pTM2 indicate putative transmembrane regions 1 and 2, respectively. The electrostatic surface is represented for a better view of the hydrophobic pTMs. b A tentative model of membrane binding by the dimeric IRGB10 via a myristoylated N-terminus and two transmembrane regions (pTMs). c Native-PAGE. The loaded protein samples are indicated above each lane. d The compact-form structure of human atlastin 1 (Atl1). The two distinct domains are colored differently, with the GTPase domain in deep blue, and the helical domain in dark pink. PPI stands for protein–protein interface. e The elongated structure of Atl1. The color code used in (d) also applies here. f Superposition between the compact form (indicated in yellow) and elongated form (in gray) of Atl1. g The structure of dimeric IRGB10. The color code used for (d) and (e) also applies here. h Concentration-dependent oligomerization of IRGB10. The profile of SEC FPLC is provided. i Putative model of IRGB10 oligomerization via structural change and the resulting membrane disruption.