Fig. 5: Verification of Mr-lcp1-122k homologue location and presence of chitin.
From: Chitin is a functional component of the larval adhesive of barnacles
a Western blot (WB) lanes containing whole cyprid extract showed a number of bands that reacted with the CBD and one band that reacted with an antibody for the N-terminal peptide of Mr-lcp1-122k (weak band, red arrow), both of which occurred at ~120 kDa. The molecular weight marker applies to both lanes. Exposures of WB were optimized for presentation purposes and the originals can be found in Supplementary Fig.s 5 and 6. b A cement gland stained using the N-terminal antibody, illustrating weak fluorescence in the β-vesicles. No fluorescence evident in negative control (scale bar: b = 20 μm).
