Fig. 4: Interaction between SCPs-A6 or G6 and LPS. | Communications Biology

Fig. 4: Interaction between SCPs-A6 or G6 and LPS.

From: Development of chimeric peptides to facilitate the neutralisation of lipopolysaccharides during bactericidal targeting of multidrug-resistant Escherichia coli

Fig. 4

a Dissociation of LPS aggregates. FITC-labeled LPS micells were incubated with A6, G6, N6, and PMB, and the fluorescent intensity was detected in a microplate reader. Results indicate means with SD (n = 3 independent experiments). b CD spectra for A6, G6, or N6 with or without E. coli LPS (0.2 mg per ml). c Binding affinity of LBP14, A6, G6, and N6 to LPS. Ampicillin (AMP) and PMB were used as negative and positive controls, respectively. Results indicate means with SD (n = 3 independent experiments). Different lower case letters indicate a difference between two groups (p < 0.05). Source data for a–c can be found in Supplementary Data 1. d, e NMR analysis of A6 (d) and G6 (e) binding to LPS. Part of the NOESY spectra for A6 and G6 showed an NOE enhancement upon addition of LPS to the peptide solvent (black: free peptide; red: peptide with LPS). Some of the tr-NOEs for the targeting domain (residues 1–14 in A6 and G6) showed that intra-residue and/or sequential NOEs were labeled beside the peaks.

Back to article page