Fig. 4: Conditional deletion of CD98hc in monocytes and macrophages leads to attenuated colitis.
a CD98hc immunofluorescence of CD98hcΔCX3CR1 mice, CD98hcflox/flox, and CD98hcΔCX3CR1 with DSS colitis treated either with corn oil or tamoxifen. b Percentage of CD98hc+ monocytes and macrophages of indicated groups 7 days after start of DSS administration. Colitis was induced by adding 2.5% dextran sodium sulfate (DSS) to CD98hcflox/flox and CD98hcΔCX3CR1 mice which were treated either with corn oil or tamoxifen 2 days before DSS administration. Each dot represents one animal. Data were analyzed by two-way ANOVA followed by Sidak’s correction; *p < 0.05, **p < 0.01, ***p < 0.001. c The mean percentage body weight change (± SD) and d disease activity index are shown. The data were analyzed by two-way ANOVA followed by Sidak’s correction; ****p < 0.0001. e Histological scores were assessed in a blinded fashion by two independent investigators. The mean histological score was determined for each animal after H&E staining of colonic tissues, presented as individual dot and analyzed with a Mann–Whitney U test; *p < 0.05. f The colon length was determined at day 7 after start of DSS administration, colon length is shown for each individual animal, the mean indicated and analyzed with a Mann–Whitney U test; *p < 0.05. g A representative image of the colon from each group is shown. h Hematoxylin staining of colonic tissues, and i endoscopic images from indicated groups. Experiments were performed four times with three to four biological replicates in each group.
