Fig. 7: Deletion of CD98hc in monocytes and macrophages leads to reduced macrophage numbers in the colonic lamina propria. | Communications Biology

Fig. 7: Deletion of CD98hc in monocytes and macrophages leads to reduced macrophage numbers in the colonic lamina propria.

From: Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice

Fig. 7: Deletion of CD98hc in monocytes and macrophages leads to reduced macrophage numbers in the colonic lamina propria.

Percentage of monocytes and macrophages that express CD98hc and total monocyte and macrophage number in the colonic lamina propria of CD98hcΔCX3CR1 mice was determined after receiving tamoxifen for 28 days. a Percentage of CD98hc+ monocytes and macrophages, and b the total monocyte and macrophage numbers in the colonic lamina propria of corn oil- and tamoxifen-treated CD98hcΔCX3CR1 animals (n = 3) are shown as the mean (± SD) and analyzed by two-way ANOVA followed by Sidak’s correction; ****p < 0.0001. c Ratios (cKO/WT cells) of total colonic lamina propria monocytes and macrophages cell numbers between corn oil and tamoxifen-treated CD98hcΔCX3CR1 at indicated time points. d Dot plots were generated by gating on CCR2+CD64−/CCR2+CD64+ and CCR2−CD64+ colonic lamina propria monocytes and macrophages isolated from tamoxifen-treated CD98hcΔCX3CR1 animals at indicated time points. Numbers show the percentage of the indicated gates. Experiments performed twice with three biological replicates in each group.

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