Fig. 10: NFIX/NORFA axis regulates porcine granulosa cell apoptosis by activating TGF-β signaling pathway.
From: NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death
a The apoptosis rate of porcine granulosa cells co-transfected with NFIX-siRNA and pcDNA3.1-NORFA were determined by FACS. b The apoptosis rate of porcine granulosa cells co-transfected with pcDNA3.1-NFIX and NORFA-siRNA were detected by FACS. c The protein levels of TGFBR2, p-SMAD3, and SMAD3 in granulosa cells after treatment with NFIX-siRNA and pcDNA3.1-NORFA were measured by western blotting. d The protein levels of TGFBR2, p-SMAD3, and SMAD3 in granulosa cells after treatment with pcDNA3.1-NFIX and NORFA-siRNA were analyzed by western blotting. Data are shown as mean ± S.E.M. with three independent experiments. P values were calculated by a two-tailed Student’s t test. *P < 0.05 and **P < 0.01.
