Fig. 4: Characterization of porcine miR-126.
From: NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death
a miR-126 mature sequences are highly conserved among vertebrates. Asterisks indicate complementarity. Red box indicate seed sequence of miR-126. ssc Sus scrofa, hsa Homo sapiens, mmu Mus musculus, rno Rattus norvegicus, gga Gallus gallus, bta Bos taurus, dre Danio rerio. b Genomic organization of miR-126 in pig and other species. The approximate distances between miRNAs and nearby protein-coding genes are given. Chromosome strands are indicated by + or −. c KEGG pathway analysis with miR-126 targets. d Heatmap of miR-126 levels during porcine ovarian follicular atresia. The data were identified by miRNA microarray assay30. HF healthy follicles, EAF early atresia follicular, PAF progressively atretic follicles. e miR-126 levels in granulosa cells from healthy follicles (HF) and atretic follicles (AF) (n = 7), analyzed by qRT-PCR. f The relationship between NORFA and miR-126 expression levels in porcine ovarian follicles was detected with Pearson analysis.
