Fig. 5: miR-126 regulates porcine granulosa cell apoptosis and mediated the functions of NORFA.
From: NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death
a, b Granulosa cells were transfected with miR-126 mimics, apoptosis rate was determined by FACS (a), and BCL-2/BAX ratio was calculated (b). c, d Granulosa cells were transfected with miR-126 inhibitor, apoptosis rate was determined by FACS (c), and BCL-2/BAX ratio was calculated (d). e, f Granulosa cells were co-transfected with pcDNA3.1-NORFA and miR-126 mimics, cell apoptosis was determined by FACS (e), and BCL-2/BAX ratio was calculated (f). g, h Granulosa cells were co-transfected with NORFA-siRNA and miR-126 inhibitor, apoptosis rate was determined by FACS (g), and BCL-2/BAX ratio was calculated (h). Data are represented as mean ± S.E.M. with three independent experiments. P value was calculated by a two-tailed Student’s t test.
