Fig. 2: Effects of removal of a single ribosomal protein from R-iSAT.

Increase in sfGFP fluorescence after 4 h incubation was normalized by dividing by the average value of the control reaction. Red bars indicate nonessential ribosomal proteins, as revealed by genome deletion studies. Orange bars indicate putative nonessential ribosomal proteins identified in this study. Error bars indicate standard deviation of triplicate measurements. Each dot represents individual observed value.