Fig. 5: Mutational analysis of uS12. | Communications Biology

Fig. 5: Mutational analysis of uS12.

From: In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli

Fig. 5

Reactions were separated into two steps: uS12 synthesis and the 30S subunit assembly were performed in the first step (2 h), and then streptomycin and sfGFP template DNA were added for sfGFP synthesis in the second step (4 h). The increase in sfGFP fluorescence after the 4-h incubation in the second step was normalized by dividing by the average value of the control reaction. Error bars indicate standard deviation of at least triplicate measurements. Each dot represents individual observed value.

Back to article page