Fig. 6: Human PBMC population phenotyping.
From: Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesis
a Phenotyping of healthy human PBMC populations using surface markers targeted using a 50-parameter antibody panel. Human PBMCs were stained with 50 TotalSeq-B antibodies and barcoded by QBC. 42,165 cells identified by data analysis of the ~26 M sequence reads. Immune cell populations were gated by traditional bivariate gating. Expression profiles and percentages are within the expected ranges for this cell type. b Expression of PBMC cell populations demonstrated via viSNE plots demonstrates appropriate cell subsets and groupings. A viSNE analysis was done for the data in Fig. 6a and a subset of the plots are shown. See Supplementary Fig. 9 for the complete set of viSNE plots. In all, 50 TotalSeq-B antibodies were used in the experiment: CD3, CD4, CD8, CD8a, TIGIT, CD274, CD27, CD28, CD25, CD137, CD19, CD20, CD24, CD45, CD45RA, CD45RO, CD56, CD69, HLA-DR, CD11b, CD14, CD11c, CD13, CD197,CD206, XCR1, CD15, CD16, CD38, CD62L, CD86, CD80, CX3CR1, CD127, CD158b, CD163, CD278, CD279, CD314, CD335, CD10, CD57, TCR α/β, TCR γ/δ, TCR Vγ9, CD155, Hashtag 1, IgG1, IgG2a, and IgG2b. viSNE analysis was done using 46 out of the 50 markers. Hashtag 1 antibody and the three isotype IgG control antibodies were left out of the clustering analysis to observe how the anti-human leukocyte markers correlated with each other.
