Fig. 3: Sh acts in GABAergic neurons to promote sleep likely via GABA transmission.
From: The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission
a A series of Gal4 drivers was genetically combined with Sh RNAi transgene (Sh RNAi#1, BL53347) to deplete endogenous Sh expression in distinct sets of cells. Sleep behaviors were analyzed individually in transgenic male flies. The differences in the averaged amount of L sleep between Gal4>ShRNAi and Gal4 control flies (∆L sleep, n = 15–91) for each Gal4 driver were plotted by blue (day 2, 21 °C) or orange dots (day 5, 29 °C), respectively. The x-axis indicates each Gal4 line sorted by the size of ∆L sleep at 21 °C. b Box plots represent the total amounts of L or D sleep on day 2 (21 °C, blue boxes) versus day 5 (29 °C, orange boxes) (n = 24–62). The Sh RNAi#1 transgene was co-expressed with Dicer-2 to enhance RNAi effects. Aligned ranks transformation ANOVA detected significant interactions of temperature with Sh depletion in all neurons (ELAV-Gal4) or GABAergic neurons (VGAT-Gal4 (II), BL58980) on L sleep (P < 0.0001). n.s., not significant; *P < 0.05, ***P < 0.001 as determined by Wilcoxon signed-rank test. c Trans-heterozygous mutations in GAD1 and VGAT suppress temperature-sensitive plasticity of wild-type and Sh mutant sleep (n = 25–122). n.s., not significant; *P < 0.05, ***P < 0.001 as determined by Aligned ranks transformation ANOVA, Wilcoxon signed-rank test.
