Fig. 1: VISUAL-CC is a new method for inducing SE–CC complexes.
From: VISUAL-CC system uncovers the role of GSK3 as an orchestrator of vascular cell type ratio in plants
a The process of vascular cell differentiation in conventional VISUAL system. b Schematic of the screening system. LUC activity of pSUC2:ELUC was monitored every 20 min during culture under various conditions. c Time course of pSUC2:ELUC intensities during culture in conventional VISUAL and VISUAL-CC. The vertical axis indicates photon counts per second detected by the luminometer. The lower panel illustrates culture conditions and medium composition during VISUAL and VISUAL-CC culture; see Materials for a detailed protocol. d, e pSUC2:YFPnls expression before (d) and after 4 days of VISUAL-CC treatment (e) in cotyledons. f Simultaneous observation of pSUC2:YFPnls expression and xylem cells after VISUAL-CC treatment. Xylem cells were detected as UV autofluorescent signal (blue). g Expression patterns of pSEOR1:SEOR1-RFP (magenta) and pSUC2:YFPnls (green) in VISUAL-CC. Cell walls were stained with calcofluor white (blue). h FE-SEM image of ectopic CC-like cells (high electron density) and SEs (low electron density) induced by VISUAL-CC. i TEM image of branched plasmodesmata between an ectopic CC-like cell and a SE. j Schematic of branched plasmodesmata. Scale bars: 500 μm (d–g); 5 μm (h); 200 nm (i).
