Fig. 3: GSK3s activity balances the SE/CC ratio in VISUAL-CC.
From: VISUAL-CC system uncovers the role of GSK3 as an orchestrator of vascular cell type ratio in plants

a Expression of SUC2 (CC) and IRX3 (XY) showing no correlation between levels in S and M samples. The Pearson correlation coefficient and P value are marked on the chart. b Negative correlation between expression of SUC2 (CC) and SEOR1 (SE). c Positive correlation between expression of SUC2 (CC) and APL (SE + CC). d Time course of pSUC2:ELUC signal intensities during VISUAL-CC with various bikinin concentrations. Averaged LUC values are shown (n = 6). e–i Expression of SUC2 (CC, e), SAPL (CC, f), SEOR1 (SE, g), CALS7 (SE, h), and DWF4 (GSK3-induced, i) in VISUAL-CC samples cultured with various bikinin concentrations for 4 days. Dots indicate relative expression levels for each sample. Statistical differences between samples are indicated by different letters (ANOVA, Tukey–Kramer method, n = 6). j Positive correlation between expression of SUC2 (CC) and DWF4 (GSK3-induced). k Negative correlation between expression of SUC2 (CC) and TCH4 (GSK3-suppressed). l Schematic model showing dose-dependent regulation of the SE/CC ratio by GSK3 activity.