Fig. 3: BMPR2-deficient adipocytes are sensitive to TNFα-induced apoptosis.
From: BMPR2 promotes fatty acid oxidation and protects white adipocytes from cell death in mice
a Levels of TNFα in serum of 14-day-old mice were determined using ELISA. n = 6. Data are expressed as means ± SD. ***p < 0.001. b Representative image of Western blot for TNFα in iWAT of two pairs of littermates at 14 days old. c Statistical analysis for the gray density of TNFα bands after normalized with HSP90 in Western blot assay described in c (n = 4). Data are expressed as means ± SD. *p < 0.05. d Western blot showed levels of cleaved caspase 3 and total caspase 3 in differentiated adipocytes from precursor cell in culture. e Image of mature adipocytes differentiated from isolated precursor cells in subcutaneous WAT at hip region of four human individuals. Scale bar: 25 μm. f Western blot showed levels of BMPR2 and cleaved caspase 3 (Cl-Caspase 3) in cells of d. g Western blot showing the levels of BMPR2 and cleaved caspase 3 in human subcutaneous adipose tissues from hip region (left panel). h Levels of BMPR2 and cleaved caspase 3 in g were normalized by HSP90 and analysis for linear regression to show their correlation (right panel).
